Human Cytosolic Sulfotransferase 2B1/SULT2B1 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB6174

R&D Systems, part of Bio-Techne
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MAB6174
MAB6174-SP
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 682516

View all Cytosolic Sulfotransferase 2B1/SULT2B1 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Cytosolic Sulfotransferase 2B1/SULT2B1
Asp2-Ser365
Accession # O00204

Specificity

Detects human Cytosolic Sulfotransferase 2B1/SULT2B1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) SULT1A1, 1A3, 1B1, 1C4, 1E1, 2A1, or 4A1 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human Cytosolic Sulfotransferase 2B1/SULT2B1 Antibody

Cytosolic Sulfotransferase 2B1/SULT2B1 antibody in Human Prostate by Immunohistochemistry (IHC-P).

Cytosolic Sulfotransferase 2B1/SULT2B1 in Human Prostate.

Cytosolic Sulfotransferase 2B1/SULT2B1 was detected in immersion fixed paraffin-embedded sections of human prostate using Mouse Anti-Human Cytosolic Sulfotransferase 2B1/SULT2B1 Monoclonal Antibody (Catalog # MAB6174) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human Cytosolic Sulfotransferase 2B1/SULT2B1 Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human prostate

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cytosolic Sulfotransferase 2B1/SULT2B1

SULT2B1 (Sulfotransferase 2B1; also ST2B1 and alcohol sulfotransferase) is a 45-48 kDa member of the sulfotransferase 1 family of enzymes. SULT2B1 is widely expressed, being found in platelets, stratum granulosum keratinocytes, breast and prostatic epithelium, and syncytiotrophoblast cells. SULT2B1 catalyzes the sulfonation of DHEA, a precursor for sex steroids, and cholesterol, which supports stratification of the epidermis. Human SULT2B1 is 365 amino acids (aa) in length. There are PAPS binding sites between aa 70-75 and 147-155, a myristoylation motif between aa 255-259, and a Pro-rich region between aa 305-364 that may extend enzyme half-life. There is one 43 kDa splice form (ST2B1a) that shows an eight aa substitution for aa 1-23. It is expressed in fetal brain and generates pregnenolone sulfate, a steroid that modulates neurotransmitters. Full-length human SULT2B1 shares 71% aa identity with mouse SULT2B1.        

Alternate Names

HSST2, SULT2B1

Entrez Gene IDs

6820 (Human); 54200 (Mouse); 292915 (Rat)

Gene Symbol

SULT2B1

UniProt

O00204 (Human)

Additional Cytosolic Sulfotransferase 2B1/SULT2B1 Products

Product Documents for Human Cytosolic Sulfotransferase 2B1/SULT2B1 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Human Cytosolic Sulfotransferase 2B1/SULT2B1 Antibody

For research use only

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