Human DAPK3/ZIPK Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5290
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human DAPK3
Arg347-Arg454
Accession # O43293
Arg347-Arg454
Accession # O43293
Specificity
Detects endogenous human DAPK3 in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human DAPK3/ZIPK Antibody
Detection of Human DAPK3/ZIPK by Western Blot.
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Human DAPK3/ZIPK Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5290) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for DAPK3/ZIPK at approximately 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.Applications for Human DAPK3/ZIPK Antibody
Application
Recommended Usage
Western Blot
0.5 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line and HeLa human cervical epithelial carcinoma cell line
Sample: HepG2 human hepatocellular carcinoma cell line and HeLa human cervical epithelial carcinoma cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: DAPK3/ZIPK
DAPK3 (Death-associated protein kinase 3; also known as ZIP kinase and DLK) is a 52 kDa member of the CAMK Ser/Thr kinase family, protein kinase superfamily of enzymes. In general, mouse DAPK3 is nuclear while human DAPK3 is cytosolic. DAPK3 is found in smooth muscle where it phosphorylates the regulatory light chain of myosin and stabilizes thick filament formation. Human DAPK3 is 454 amino acids (aa) in length and contains one protein kinase domain (aa 134‑275) and a C-terminal Leu-zipper motif (aa 427‑441) that mediates homo- and hetero-dimerization. There is one 35 kDa alternate splice form that shows a 10 aa substitution for aa 313‑454. This form has both a nuclear and cytosolic localization.
Long Name
Death-associated Protein Kinase 3
Alternate Names
Dlk, ZIPK
Gene Symbol
DAPK3
UniProt
Additional DAPK3/ZIPK Products
Product Documents for Human DAPK3/ZIPK Antibody
Product Specific Notices for Human DAPK3/ZIPK Antibody
For research use only
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