Human DC-STAMP APC-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB7824A
Conjugate
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Flow Cytometry
Cited:
Flow Cytometry
Label
Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)
Antibody Source
Monoclonal Mouse IgG2B Clone # 788524
Product Specifications
Immunogen
E. coli-derived recombinant human DC-STAMP
Asp314-Thr376
Accession # Q9H295
Asp314-Thr376
Accession # Q9H295
Specificity
Detects human DC-STAMP in direct ELISAs.
In direct ELISAs, no cross-reactivity
with recombinant mouse DC-STAMP is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human DC-STAMP APC-conjugated Antibody
Detection of DC-STAMP in Human Denditic Cells by Flow Cytometry.
Mature human monocyte-derived dendritic cells treated with 20 ng/mL Recombinant Human IL-4 (Catalog # 204-IL) and 50 ng/mLRecombinant Human GM-CSF (Catalog # 215-GM) for 6 days then treated with 20 ng/mL LPS, 20 ng/mL Recombinant Human TNF-a (Catalog # 210-TA), and 20 ng/ml Recombinant Human IL-1 beta (Catalog # 201-LB) for 24 hourswere stained with Mouse Anti-Human CD83 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB1774F) and either (A) Mouse Anti-Human DC-STAMP APC-conjugated Monoclonal Antibody (Catalog # FAB7824A) or (B) Mouse IgG2BAllophycocyanin Isotype Control (Catalog # IC0041A). View our protocol for Staining Membrane-associated Proteins.Applications for Human DC-STAMP APC-conjugated Antibody
Application
Recommended Usage
Flow Cytometry
10 µL/106 cells
Sample: Human monocyte-derived dendritic cells treated with Recombinant Human IL-4 (Catalog # 204-IL) and Recombinant Human GM-CSF (Catalog # 215-GM) for 6 days then treated with LPS, Recombinant Human TNF-alpha (Catalog # 210-TA), and Recombinant Human IL-1 beta (Catalog # 201-LB) for 24 hours
Sample: Human monocyte-derived dendritic cells treated with Recombinant Human IL-4 (Catalog # 204-IL) and Recombinant Human GM-CSF (Catalog # 215-GM) for 6 days then treated with LPS, Recombinant Human TNF-alpha (Catalog # 210-TA), and Recombinant Human IL-1 beta (Catalog # 201-LB) for 24 hours
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: DC-STAMP
DC-STAMP, also known as TM7SF4, is an approximately 50 kDa glycoprotein with seven transmembrane segments. It is expressed on the surface of dendritic cells and monocytes as well as on osteoclasts and their progenitors. DC-STAMP binds CTGF/CCN2 and cooperates with TRANCE/RANK L for inducing osteoclast differentiation and fusion into multinucleated cells. It contains a cytoplasmic immunoreceptor tyrosine-based inhibitory motif (ITIM) and associates with FC gamma RIII/CD16. Within aa 314-376, human DC-STAMP shares approximately 75% aa sequence identity with mouse and rat DC-STAMP.
Long Name
Dendritic Cell-specific Transmembrane Protein
Alternate Names
DCSTAMP, FIND, IL-4-induced Protein, TM7SF4
Gene Symbol
DCSTAMP
UniProt
Additional DC-STAMP Products
Product Documents for Human DC-STAMP APC-conjugated Antibody
Product Specific Notices for Human DC-STAMP APC-conjugated Antibody
For research use only
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