Human DEC-205/CD205 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB20471
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 523218
Product Specifications
Immunogen
E. coli-derived recombinant human DEC‑205/CD205
Cys216-Cys501
Accession # O60449
Cys216-Cys501
Accession # O60449
Specificity
Detects human DEC‑205/CD205 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse DEC-205 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human DEC-205/CD205 Antibody
Detection of Human DEC-205/CD205 by Western Blot.
Western blot shows lysates of in vitro differentiated human immature myeloid dendritic cells. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human DEC-205/CD205 Monoclonal Antibody (Catalog # MAB20471) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for DEC-205/CD205 at approximately 200 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Western Blot Buffer Group 1.
Detection of Human DEC-205/CD205 by Western Blot.
Western blot shows lysates of human thymus. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human DEC-205/CD205 Monoclonal Antibody (Catalog # MAB20471) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF007). A specific band was detected for DEC-205/CD205 at approximately 250 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Western Blot Buffer Group 1.Applications for Human DEC-205/CD205 Antibody
Application
Recommended Usage
Western Blot
2 µg/mL
Sample: In vitro differentiated human immature myeloid dendritic cells and human thymus
Sample: In vitro differentiated human immature myeloid dendritic cells and human thymus
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: DEC-205/CD205
Alternate Names
CD205, DEC205, Ly75
Gene Symbol
LY75
UniProt
Additional DEC-205/CD205 Products
Product Documents for Human DEC-205/CD205 Antibody
Product Specific Notices for Human DEC-205/CD205 Antibody
For research use only
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