Human DEC-205/CD205 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF2047
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human DEC‑205/CD205
Cys216-Cys501
Accession # O60449
Cys216-Cys501
Accession # O60449
Specificity
Detects human DEC‑205/CD205 in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human DEC-205/CD205 Antibody
Detection of Human DEC‑205/CD205 by Western Blot.
Western blot shows lysates of human thymus tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human DEC-205/CD205 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2047) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for DEC-205/ CD205 at approximately 250 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
DEC‑205/CD205 in Human PBMCs.
DEC-205/CD205 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using 10 µg/mL Goat Anti-Human DEC-205/CD205 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2047) for 3 hours at room temperature. Cells were stained with the Northern-Lights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.Applications for Human DEC-205/CD205 Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed human peripheral blood mononuclear cells (PBMCs)
Sample: Immersion fixed human peripheral blood mononuclear cells (PBMCs)
Western Blot
1 µg/mL
Sample: Human thymus tissue
Sample: Human thymus tissue
Reviewed Applications
Read 1 review rated 4 using AF2047 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: DEC-205/CD205
Alternate Names
CD205, DEC205, Ly75
Gene Symbol
LY75
UniProt
Additional DEC-205/CD205 Products
Product Documents for Human DEC-205/CD205 Antibody
Product Specific Notices for Human DEC-205/CD205 Antibody
For research use only
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