Human peripheral blood lymphocytes were stained with Mouse Anti-Human DEP-1/CD148 PE-conjugated Monoclonal Antibody (Catalog # FAB1934P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human DEP-1/CD148 PE-conjugated Antibody

Application

Recommended Usage

Flow Cytometry

10 µL/10⁶ cells
Sample: Human peripheral blood lymphocytes

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: DEP-1/CD148

Density Enhanced Protein Tyrosine Phosphatase (DEP-1), also known as CD148, HPTP-eta, and PTP Receptor Type J (PTPRJ), is an enzyme that removes phosphate groups covalently attached to tyrosine residues in proteins. A large (220 kDa) glycoprotein found at the cell surface, DEP-1 levels are increased with high cell density (1). DEP-1 phosphatase activity is enhanced by basement membrane proteins (2), suggesting it is involved in regulating cell adhesion and contact interactions. High levels of expression dampen PDGF (3), VEGF (4), and T-cell receptor (5) responses. DEP-1 is widely expressed in tissues, particularly ones forming epithelioid monolayers (6). In the immune system, DEP-1 is found on all cell lineages and is highest on granulocytes (7). Dep-1 is the mutated gene in the Susceptibility to Colon Cancer locus Scc1, which is altered in many human colorectal adenomas (8). Gene knockout mice lacking DEP-1 die at midgestation due to failures in cardiovascular development (9). DEP-1 dephosphorylates a variety of proteins, including the HGF (10), PDGF (11), and VEGF (4) receptors, and beta-Catenin (12). The recombinant protein is the intracellular region of DEP-1 containing the catalytic domain.

References

Ostman, A. et al. (1994) Proc. Natl. Acad. Sci. USA 91:9680.
Sorby, M. et al. (2001) Oncogene 20:5219.
Jandt, E. et al. (2003) Oncogene 22:4175.
Lampugnani, M.G. et al. (2003) J. Cell Biol. 161:793.
Baker, J.E. et al. (2001) Mol. Cell. Biol. 21:2393.
Borges, L.G. et al. (1996) Circ. Res. 79:570.
de la Fuente-Garcia, M.A. et al. (1998) Blood 91:2800.
Ruivenkamp, C.A. et al. (2002) Nat. Genet. 31:295.
Takahashi, T. et al. (2003) Mol. Cell. Biol. 23:1817.
Palka, H.L. et al. (2003) J. Biol. Chem. 278:5728.
Kovalenko, M. et al. (2000) J. Biol. Chem. 275:16219.
Holsinger, L.J. et al. (2002) Oncogene 21:7067.

Long Name

Density Enhanced Receptor Protein Tyrosine Phosphatase

Alternate Names

CD148, DEP1, HPTP-eta, PTPRJ

Entrez Gene IDs

5795 (Human); 19271 (Mouse); 29645 (Rat)

Gene Symbol

PTPRJ

Additional DEP-1/CD148 Products

Product Documents for Human DEP-1/CD148 PE-conjugated Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Human DEP-1/CD148 PE-conjugated Antibody

For research use only

Citations
Reviews

Spectra Viewer
Flow Cytometry

Human DEP-1/CD148 PE-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB1934P

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

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Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human DEP-1/CD148 PE-conjugated Antibody

Detection of DEP‑1/CD148 in Human Blood Lymphocytes by Flow Cytometry.