Human Desmoglein-3 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB1720
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry, Western Blot
Cited:
Flow Cytometry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 216519
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Desmoglein-3
Glu50-Arg615
Accession # P32926
Glu50-Arg615
Accession # P32926
Specificity
Detects human Desmoglein-3 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) Desmoglein-1, rhDesmoglein-2, or rhCadherin-17 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human Desmoglein-3 Antibody
Detection of Human Desmoglein‑3 by Western Blot.
Western blot shows lysates of human skin cancer (squamous cell carcinoma) tissue. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human Desmoglein-3 Monoclonal Antibody (Catalog # MAB1720) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Desmoglein-3 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Desmoglein‑3 in Human Squamous Cell Carcinoma.
Desmoglein-3 was detected in immersion fixed paraffin-embedded sections of human squamous cell carcinoma using Mouse Anti-Human Desmoglein-3 Monoclonal Antibody (Catalog # MAB1720) at 8 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membranes of keratinocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human Desmoglein-3 Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human skin and human squamous cell carcinoma
Sample: Immersion fixed paraffin-embedded sections of human skin and human squamous cell carcinoma
Western Blot
0.5 µg/mL
Sample: Human skin cancer (squamos cell)
Sample: Human skin cancer (squamos cell)
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Desmoglein-3
References
- Kottke, M.D. et al. (2006) J. Cell Sci. 119:797.
- Garrod, D.R. et al. (2002) Mol. Membr. Biol. 19:81.
- Leckband, D. and A. Prakasam (2006) Annu. Rev. Biomed. Eng. 8:259.
- Amagai, M. et al. (1991) Cell 67:869.
- Silos, S.A. et al. (1996) J. Biol. Chem. 271:17504.
- Amagai, M. et al (1996) J. Invest. Dermatol. 106:351.
- Weiske, J. et al. (2001) J. Biol. Chem. 276:41175.
- Cirillo, N. et al. (2007) Oral Dis. 13:341.
- Wang, L. et al. (2007) Virchows Arch. Sept. 11 epub.
- Calkins, C.C. et al. (2006) J. Biol. Chem. 281:7623.
Alternate Names
CDHF6, Desmoglein3, DSG3
Gene Symbol
DSG3
UniProt
Additional Desmoglein-3 Products
Product Documents for Human Desmoglein-3 Antibody
Product Specific Notices for Human Desmoglein-3 Antibody
For research use only
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