Human Desmoplakin Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB9080

R&D Systems, part of Bio-Techne
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MAB9080
MAB9080-SP
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 824038

View all Desmoplakin Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Desmoplakin
Asn1796-Val2046
Accession # P15924

Specificity

Detects human Desmoplakin in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Desmoplakin Antibody

Detection of Human Desmoplakin antibody by Western Blot.

Detection of Human Desmoplakin by Western Blot.

Western blot shows lysate of COLO 205 human colorectal adenocarcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human Desmoplakin Monoclonal Antibody (Catalog # MAB9080) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for Desmoplakin at approximately 260 and 330 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human Desmoplakin Antibody

Application
Recommended Usage

Western Blot

0.5 µg/mL
Sample: COLO 205 human colorectal adenocarcinoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Desmoplakin

DSP (desmoplakin) is the major high molecular weight protein of desmosomes, the most common type of intercellular junction in vertebrate epithelial cells.  DSP is involved in the organization of the desmosomal cadherin-plakoglobin complex, as well as in the anchoring of intermediate filaments to desmosomes.  These functions are thought to impart tensile strength and integrity to epithelial and vascular tissues.  Mutations in DSP result in several clinical phenotypes, ranging from mild skin conditions, to severe skin blistering with subsequent dehydration, to lethal heart defects.  Two major desmoplakin isoforms, DSPI and DSPII, are encoded by alternative mRNA transcripts differentially spliced from the same gene.  Human DSPI is 2,871 amino acids (aa) in length, while DSPII lacks part of a
central alpha-helical rod domain, and is 599 aa shorter.

Alternate Names

DCWHKTA, DPI, DPII, DSP, KPPS2, PPKS2

Entrez Gene IDs

1832 (Human); 109620 (Mouse); 306871 (Rat)

Gene Symbol

DSP

UniProt

P15924

Additional Desmoplakin Products

Product Documents for Human Desmoplakin Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Desmoplakin Antibody

For research use only

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