Human DFF40/CAD Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB6289
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunocytochemistry, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 609809
Product Specifications
Immunogen
E. coli-derived recombinant human DFF40/CAD
Met1-Gln338
Accession # O76075
Met1-Gln338
Accession # O76075
Specificity
Detects human DFF40/CAD in direct ELISAs and Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human DFF40/CAD Antibody
Detection of Human DFF40/CAD by Western Blot.
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and Jurkat human acute T cell leukemia cell line. PVDF Membrane was probed with 0.5 µg/mL of Mouse Anti-Human DFF40/CAD Monoclonal Antibody (Catalog # MAB6289) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for DFF40/CAD at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
DFF40/CAD in HeLa Human Cell Line.
DFF40/CAD was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human DFF40/CAD Monoclonal Antibody (Catalog # MAB6289) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Mouse IgG Secondary Antibody (green; Catalog # NL009) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human DFF40/CAD Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line
Western Blot
0.5 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line and Jurkat human acute T cell leukemia cell line
Sample: HepG2 human hepatocellular carcinoma cell line and Jurkat human acute T cell leukemia cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: DFF40/CAD
DNA fragmentatation factor 40 kDa subunit (DFF40), also known as CAD, is a member of the CIDE domain-containing proteins. It is an apoptotic nuclease that is maintained in an inactive state by association with DFF45. Apoptotic signaling induces DFF45 cleavage and the release of DFF40. Active DFF40 exists as an oligomer and generates double stranded breaks in dsDNA. Alternate splicing of human DFF40 generates isoforms with substitutions and truncations either directly following the CIDE domain (which is aa 4‑80) or at the C-terminus. Full length human DFF40 (aa 1‑338) shares 76% and 74% aa sequence identity with mouse and rat DFF40, respectively.
Long Name
DNA Fragmentation Factor, Subunit beta
Alternate Names
CAD, CPAN, DFF2, DFFB
Gene Symbol
DFFB
UniProt
Additional DFF40/CAD Products
Product Documents for Human DFF40/CAD Antibody
Product Specific Notices for Human DFF40/CAD Antibody
For research use only
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