Human DNA-PKcs Antibody
R&D Systems, part of Bio-Techne | Catalog # AF3415
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human DNA-PKCS
Thr3844-Trp4127
Accession # P78527
Thr3844-Trp4127
Accession # P78527
Specificity
Detects human DNA-PKCS in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human DNA-PKcs Antibody
Detection of Human DNA-PKcs by Western Blot.
Western blot shows lysates of U2OS human osteosarcoma cell line and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Human DNA-PKcs Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3415) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for DNA-PKcs at approximately 460 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human DNA-PKcs Antibody
Application
Recommended Usage
Western Blot
0.5 µg/mL
Sample: U2OS human osteosarcoma cell line and HeLa human cervical epithelial carcinoma cell line
Sample: U2OS human osteosarcoma cell line and HeLa human cervical epithelial carcinoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: DNA-PKcs
DNA-dependent protein kinase catalytic subunit (DNA-PKCS) is a member of the PI3-kinase related kinase (PIKK) superfamily. DNA-PKCS is a serine/threonine kinase that, along with the DNA-binding proteins Ku70/Ku80, binds to regions of DNA double-strand breaks and is involved in double-strand break repair and VDJ recombination.
Long Name
DNA-dependent Protein Kinase Catalytic Subunit
Alternate Names
DNAPK, DNAPKcs, DNPK1, HYRC1, p350, PRKDC, XRCC7
Gene Symbol
PRKDC
UniProt
Additional DNA-PKcs Products
Product Documents for Human DNA-PKcs Antibody
Product Specific Notices for Human DNA-PKcs Antibody
For research use only
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