Human DOK3 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB4795
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 642316
Product Specifications
Immunogen
E. coli-derived recombinant human DOK3
Gly181-Lys271
Accession # Q7L591
Gly181-Lys271
Accession # Q7L591
Specificity
Detects human DOK3 in direct ELISAs and Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human DOK3 Antibody
Detection of Human DOK3 by Western Blot.
Western blot shows lysates of human peripheral blood mononuclear cells (PBMC). PVDF Membrane was probed with 0.25 µg/mL of Mouse Anti-Human DOK3 Monoclonal Antibody (Catalog # MAB4795) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for DOK3 at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.Applications for Human DOK3 Antibody
Application
Recommended Usage
Western Blot
0.25 µg/mL
Sample: Human peripheral blood mononuclear cells (PBMC)
Sample: Human peripheral blood mononuclear cells (PBMC)
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: DOK3
DOK3 (downstream of kinase 3) is a 53 kDa (predicted) member of the DOK family of cytoplasmic adaptor proteins and is expressed in B cells and macrophages. Human DOK3 cDNA encodes 496 amino acids (aa) including one pleckstrin homology region (aa 63‑179) and an IRS-type phosphotyrosine binding domain (PTB) (aa 213‑317). Alternate splicing generates 330, 228 and 216 aa isoforms with N-terminal and C-terminal truncations and substitutions. Human DOK3 shares approximately 80% aa sequence identity with mouse and rat DOK3 within aa 181‑271, which are common to all four isoforms. Phosphorylation of tyrosine residues in DOK3 regulates its interaction with SH2 and SH3 containing proteins, leading to inhibition of signaling from immunoreceptors.
Long Name
Downstream of Tyrosine Kinase 3
Alternate Names
docking protein 3, DOKL, Dok-like protein, Downstream of tyrosine kinase 3, FLJ22570, FLJ39939
Gene Symbol
DOK3
UniProt
Additional DOK3 Products
Product Documents for Human DOK3 Antibody
Product Specific Notices for Human DOK3 Antibody
For research use only
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