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Human Dopamine D5R/DRD5 Alexa Fluor® 647-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB82861R

R&D Systems, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Alexa Fluor 647 (Excitation = 650 nm, Emission = 668 nm)

Antibody Source

Monoclonal Mouse IgG2A Clone # 889022

Product Specifications

Immunogen

NS0 mouse myeloma cell line transfected with human Dopamine D5 R/DRD5
Met1-His477
Accession # P21918

Specificity

Detects HEK293 human embryonic kidney cell line transfected with human Dopamine D5 R/DRD5 by Flow Cytometry. Does not detect untransfected or irrelevant transfected HEK293 cells.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Applications for Human Dopamine D5R/DRD5 Alexa Fluor® 647-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

0.25-1 µg/106 cells
Sample: HEK293 human embryonic kidney cell line transfected with human Dopamine D5 R/DRD5 and eGFP
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Dopamine D5R/DRD5

DRD5, also known as Dopamine D5 Receptor has a higher affinity for neurotransmitter dopamine than DRD1 (1). DRD5 is expressed in neurons in many human brain regions, including cortex regions, hippocampus, choroid plexus, and brainstem (2). Polymorphisms in the DRD5 gene have been associated with Attention Deficit Hyperactivity Disorder (ADHD) (3), schizopherenia (4) and nicotine dependence (5). Dopamine receptors undergo endocytosis upon interaction with receptor agonists and by activation of Protein Kinase C (PKC) (6).

References

  1. Sunahra, R. et al. (1991) Nature. 350:614.
  2. Weinshank, R. et al. (1991) J Biol Chem. 266:22427.
  3. Squassina, A. et al.  (2008) Neurosci Lett. Feb 13.
  4. Golimbet, V. et al.  (2008) Bull Exp Biol Med. Jan.
  5. Wei, J. et al.  (2012) Addict Behav. 37:622.
  6. Thompson, D. et al. (2011) Traffic. 12:644.

Long Name

Dopamine D5 Receptor

Alternate Names

DBDR, Dopamine D5 R, DRD1B, DRD1L2, DRD5

Entrez Gene IDs

1816 (Human); 13492 (Mouse); 25195 (Rat)

Gene Symbol

DRD5

UniProt

Additional Dopamine D5R/DRD5 Products

Product Documents for Human Dopamine D5R/DRD5 Alexa Fluor® 647-conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Dopamine D5R/DRD5 Alexa Fluor® 647-conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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