Human Dopamine D5R/DRD5 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB82861

R&D Systems, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Applications

CyTOF-ready, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 889022

View all Dopamine D5R/DRD5 Antibodies »

Product Summary for Human Dopamine D5R/DRD5 Antibody

Immunogen

NS0 mouse myeloma cell line transfected with human Dopamine D5 R/DRD5
Met1-His477
Accession # P21918

Specificity

Detects HEK293 human embryonic kidney cell line transfected with human Dopamine D5 R/DRD5 by Flow Cytometry. Does not detect untransfected or irrelevant transfected HEK293 cells.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human Dopamine D5R/DRD5 Antibody

Detection of Dopamine D5 R/DRD5 antibody in HEK293 Human Cell Line Transfected with Human Dopamine D5 R/DRD5 and eGFP antibody by Flow Cytometry.

Detection of Dopamine D5 R/DRD5 in HEK293 Human Cell Line Transfected with Human Dopamine D5 R/DRD5 and eGFP by Flow Cytometry.

HEK293 human embryonic kidney cell line transfected with either (A) human Dopamine D5 R/DRD5 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human Dopamine D5 R/DRD5 Monoclonal Antibody (Catalog # MAB82861) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB003).

Applications for Human Dopamine D5R/DRD5 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: HEK293 human embryonic kidney cell line transfected with human Dopamine D5 R/DRD5 and eGFP
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 4 using MAB82861 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Dopamine D5R/DRD5

DRD5, also known as Dopamine D5 Receptor has a higher affinity for neurotransmitter dopamine than DRD1 (1). DRD5 is expressed in neurons in many human brain regions, including cortex regions, hippocampus, choroid plexus, and brainstem (2). Polymorphisms in the DRD5 gene have been associated with Attention Deficit Hyperactivity Disorder (ADHD) (3), schizopherenia (4) and nicotine dependence (5). Dopamine receptors undergo endocytosis upon interaction with receptor agonists and by activation of Protein Kinase C (PKC) (6).

References

  1. Sunahra, R. et al. (1991) Nature. 350:614.
  2. Weinshank, R. et al. (1991) J Biol Chem. 266:22427.
  3. Squassina, A. et al.  (2008) Neurosci Lett. Feb 13.
  4. Golimbet, V. et al.  (2008) Bull Exp Biol Med. Jan.
  5. Wei, J. et al.  (2012) Addict Behav. 37:622.
  6. Thompson, D. et al. (2011) Traffic. 12:644.

Long Name

Dopamine D5 Receptor

Alternate Names

DBDR, Dopamine D5 R, DRD1B, DRD1L2, DRD5

Entrez Gene IDs

1816 (Human); 13492 (Mouse); 25195 (Rat)

Gene Symbol

DRD5

UniProt

P21918

Additional Dopamine D5R/DRD5 Products

Product Documents for Human Dopamine D5R/DRD5 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Dopamine D5R/DRD5 Antibody

For research use only

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