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Human DPCR-1 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB33471

R&D Systems, part of Bio-Techne
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MAB33471-100
MAB33471-SP

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 986124

Product Specifications

Immunogen

Human DPCR-1 synthetic peptide
Accession # Q3MIW9

Specificity

Detects human DPCR-1 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human DPCR-1 Antibody

DPCR-1 antibody in HT-29 Human Cell Line by Immunocytochemistry (ICC).

DPCR‑1 in HT‑29 Human Cell Line.

DPCR-1 was detected in immersion fixed HT-29 human colon adenocarcinoma cell line (positive control) and not U-87 MG human glioblastoma/astrocytoma cell line (negative control) using Mouse Anti-Human DPCR-1 Monoclonal Antibody (Catalog # MAB33471) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human DPCR-1 Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed HT‑29 human colon adenocarcinoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DPCR-1

DPCR-1 (Diffuse Panbronchiolitis Critical Region 1) is encoded by a gene that maps to the chromosomal diffuse panbronchiolitis critical region. DPCR-1 is associated with susceptibility to this bronchiolar disease. It has homology with the mucin-like domain of zonadhesin. DPCR-1 is predominantly expressed in lung, kidney, and testis.

Long Name

Diffuse Panbronchiolitis Critical Region 1

Alternate Names

DPCR1, PBLT

Entrez Gene IDs

135656 (Human); 268949 (Mouse); 406199 (Rat)

Gene Symbol

MUCL3

UniProt

Additional DPCR-1 Products

Product Documents for Human DPCR-1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human DPCR-1 Antibody

For research use only

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