Human DPP6 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF2360
Key Product Details
Species Reactivity
Human
Applications
CyTOF-ready, Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human DPP6
Thr56-Asp803 (Glu458Val)
Accession # AAA35761
Thr56-Asp803 (Glu458Val)
Accession # AAA35761
Specificity
Detects human DPP6 in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human DPP6 Antibody
DPP6 in Human Spinal Cord.
DPP6 was detected in immersion fixed paraffin-embedded sections of human spinal cord using Goat Anti-Human DPP6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2360) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling when primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of DPP6 in Human SHSY5Y Cell Line by Flow Cytometry.
Human SHSY5Y neuroblastoma cell line was stained with Goat Anti-Human DPP6 Polyclonal Antibody (Catalog # AF2360, filled histogram) or control antibody (AB-108-C, open histogram), followed by APC-conjugated Anti-Goat IgG Secondary Antibody (F0108). Staining was performed using our Staining Membrane Proteins protocol.Applications for Human DPP6 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
0.25 µg/106 cells
Sample: SHSY5Y human neuroblastoma cell line or human differentiated neuron progenitor cells
Sample: SHSY5Y human neuroblastoma cell line or human differentiated neuron progenitor cells
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human spinal cord
Sample: Immersion fixed paraffin-embedded sections of human spinal cord
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human DPP6, see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Human DPP6, see our available Western blot detection antibodies
Western Blot
0.1 µg/mL
Sample: Recombinant Human DPP6
Sample: Recombinant Human DPP6
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: DPP6
Long Name
Dipeptidyl-peptidase 6
Alternate Names
DPPX
Entrez Gene IDs
1804 (Human)
Gene Symbol
DPP6
UniProt
Additional DPP6 Products
Product Documents for Human DPP6 Antibody
Product Specific Notices for Human DPP6 Antibody
For research use only
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