Human DPP9 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB5419
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunocytochemistry, Simple Western, Western Blot
Cited:
Immunoprecipitation
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 757004
Product Specifications
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human DPP9
Arg2-Leu892 (predicted)
Accession # Q1ZZB8
Arg2-Leu892 (predicted)
Accession # Q1ZZB8
Specificity
Detects human DPP9 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity
with recombinant human (rh) DPPIV/CD26 or rhDPP10 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human DPP9 Antibody
Detection of Human DPP9 by Western Blot.
Western blot shows lysates of human liver tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human DPP9 Monoclonal Antibody (Catalog # MAB5419) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for DPP9 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
DPP9 in HeLa Human Cell Line.
DPP9 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human DPP9 Monoclonal Antibody (Catalog # MAB5419) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of Human DPP9 by Simple WesternTM.
Simple Western lane view shows lysates of human liver tissue, loaded at 0.5 mg/mL. A specific band was detected for DPP9 at approximately 101 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human DPP9 Monoclonal Antibody (Catalog # MAB5419). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.Applications for Human DPP9 Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line
Simple Western
10 µg/mL
Sample: Human liver tissue
Sample: Human liver tissue
Western Blot
1 µg/mL
Sample: Human liver tissue
Sample: Human liver tissue
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: DPP9
DPP9 is a member of the S9b family of serine peptidases (1, 2). It shares 19% amino acid identity with DPP4 and 58% amino acid identity with DPP8. It exhibits post‑proline dipeptidyl aminopeptidase activity, cleaving Xaa-Pro dipeptides from the N-terminus of oligo- and polypeptides (3). Unlike DPP4, DPP9 does not appear to be membrane bound and is localized exclusively in the cytoplasm (4). This family of proline-specific dipeptidyl peptidases has been implicated in a variety of diseases including type 2 diabetes, obesity and cancer, and has been a potential target for drug discovery (5, 6).
References
- Olsen, C. and Wagtmann, N. (2002) Gene 299:185.
- Qi, S.Y. et al. (2003) Biochem. J. 373:179.
- Bjelke, J.R. et al. (2006) Biochem. J. 396:391.
- Ajami, K. et al. (2004) Biochim. Biophys. Acta. 1679:18.
- Rosenblum, J.S. and Kozarich, J.W. et al. (2003) Curr. Opin. Chem. Biol. 7:496.
- Van der Veken, P. et al. (2007) Curr. Top. Med. Chem. 7:621.
Long Name
Dipeptidyl peptidase 9
Alternate Names
DPLP9, DPRP2
Gene Symbol
DPP9
UniProt
Additional DPP9 Products
Product Documents for Human DPP9 Antibody
Product Specific Notices for Human DPP9 Antibody
For research use only
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