Human DPPA2 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF7086
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human DPPA2
Ser2-Thr174
Accession # Q7Z7J5
Ser2-Thr174
Accession # Q7Z7J5
Specificity
Detects human DPPA2 in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human DPPA2 Antibody
DPPA2 in BG01V Human Embryonic Stem Cells.
DPPA2 was detected in immersion fixed BG01V human embryonic stem cells using Sheep Anti-Human DPPA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7086) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; Catalog # NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human DPPA2 Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed BG01V human embryonic stem cells
Sample: Immersion fixed BG01V human embryonic stem cells
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: DPPA2
DPPA2 (Developmental pluripotency associated 2; also ESCA, CT100 and PESCRG1) is a 38-39 kDa member of both the DPPA and cancer-testis family of proteins. It is found in pluripotent stem cells in a pattern that parallels that of Oct4, and likely contributes to formation of the ICM, plus the maintainence of cells in an undifferentiated state. DPPA2 is also found in spermatocytes and select tumor types. Human DPPA2 is 298 amino acids (aa) in length. It contains one DNA-binding SAP domain that is involved in chromosomal organization (aa 92-126). One potential isoform variant has been reported that shows a deletion of aa 61-71. Over aa 2‑174, human DPPA2 shares only 36% aa identity with mouse DPPA2.
Long Name
Developmental Pluripotency Associated Protein 2
Alternate Names
CT100, ECSA, PESCRG1
Gene Symbol
DPPA2
UniProt
Additional DPPA2 Products
Product Documents for Human DPPA2 Antibody
Product Specific Notices for Human DPPA2 Antibody
For research use only
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