Human DPPA5/ESG1 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF3125

R&D Systems, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
AF3125
AF3125-SP
Print Quote
Bulk Order
Conjugate
Catalog #
Biotin
BAF3125
View Product
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunocytochemistry, Western Blot

Cited:

Immunocytochemistry, Immunohistochemistry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

View all DPPA5/ESG1 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human DPPA5/ESG1
Met1-Lys116
Accession # NP_001020461

Specificity

Detects human DPPA5/ESG1 in direct ELISAs and Western blots. In Western blots, approximately 40% cross-reactivity with recombinant mouse DPPA5 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human DPPA5/ESG1 Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed NTera-2 human testicular embryonic carcinoma cell line

Western Blot

0.1 µg/mL
Sample: Recombinant Human DPPA5/ESG1

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DPPA5/ESG1

DPPA5, also known as embryonic stem cell-specific gene 1 (ESG1), is a K homology RNA-binding (KH) domain containing protein that is expressed only in early embryos, germ cells and embryonic stem cells. It may be useful as a marker of pluripotent stem cells. Human DPPA5 shares approximately 66% and 74% amino acid sequence homology with mouse and rat DPPA5, respectively.

Long Name

Developmental Pluripotency Associated 5

Alternate Names

ESG1

Entrez Gene IDs

340168 (Human); 434423 (Mouse); 301101 (Rat)

Gene Symbol

DPPA5

UniProt

NP_001020461

Additional DPPA5/ESG1 Products

Product Documents for Human DPPA5/ESG1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human DPPA5/ESG1 Antibody

For research use only

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.