Human DRAK1 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB4985

R&D Systems, part of Bio-Techne
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MAB4985
MAB4985-SP
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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 684324

View all DRAK1 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human DRAK1
Ala115-Glu313
Accession # Q9UEE5

Specificity

Detects human DRAK1 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) DRAK2, rhDAPK1, rhDAPK2, or rhDAPK3 is observed. In Western blots, no cross-reactivity with rhDRAK2 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human DRAK1 Antibody

Detection of Human DRAK1 antibody by Western Blot.

Detection of Human DRAK1 by Western Blot.

Western blot shows lysates of MOLT-4 human acute lymphoblastic leukemia cell line and A431 human epithelial carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human DRAK1 Monoclonal Antibody (Catalog # MAB4985) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for DRAK1 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Applications for Human DRAK1 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: MOLT‑4 human acute lymphoblastic leukemia cell line and A431 human epithelial carcinoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DRAK1

DRAK1 (DAP kinase-related apoptosis-inducing protein kinase 1; also STK17A) is a 55 kDa member of the DAP kinase subfamily, CAMK Ser/Thr protein kinase family of enzymes. It is expressed in osteoclasts, resides in the nucleus, and initiates apoptosis. Human DRAK1 is 414 amino acids (aa) in length. It contains an N-terminal polyPro segment (aa 33-37) followed by a Ser/Thr kinase domain (aa 64-321) with a catalytic loop (aa 184-193). Apoptosis apparently requires an intact kinase domain. No rodent DRAK1 has been reported. Over aa 115-313, human DRAK1 shares 95% aa identity with canine DRAK1, and 65% aa identity with human DRAK2.      

Long Name

DAP Kinase-related Apoptosis-inducing Protein Kinase 1

Alternate Names

STK17A

Entrez Gene IDs

9263 (Human)

Gene Symbol

STK17A

UniProt

Q9UEE5

Additional DRAK1 Products

Product Documents for Human DRAK1 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human DRAK1 Antibody

For research use only

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