Human DRAK1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4985
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human DRAK1
Ala115-Glu313
Accession # Q9UEE5
Ala115-Glu313
Accession # Q9UEE5
Specificity
Detects human DRAK1 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross‑reactivity with recombinant human DRAK2 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human DRAK1 Antibody
Detection of Human DRAK1 by Western Blot.
Western blot shows lysates of JEG-3 human epithelial choriocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human DRAK1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4985) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for DRAK1 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human DRAK1 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: JEG-3 human epithelial choriocarcinoma cell line
Sample: JEG-3 human epithelial choriocarcinoma cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: DRAK1
DRAK1 (DAP kinase-related apoptosis-inducing protein kinase 1; also STK17A) is a 55 kDa member of the DAP kinase subfamily, CAMK Ser/Thr protein kinase family of enzymes. It is expressed in osteoclasts, resides in the nucleus and initiates apoptosis. Human DRAK1 is 414 amino acids (aa) in length. It contains an N‑terminal polyPro segment (aa 33‑37) followed by a Ser/Thr kinase domain (aa 64‑321) with a catalytic loop (aa 184‑193). Apoptosis apparently requires an intact kinase domain. No rodent DRAK1 has been reported. Over aa 115‑313, human DRAK1 is 95% aa identical to canine DRAK1, and 65% aa identical to human DRAK2.
Long Name
DAP Kinase-related Apoptosis-inducing Protein Kinase 1
Alternate Names
STK17A
Entrez Gene IDs
9263 (Human)
Gene Symbol
STK17A
UniProt
Additional DRAK1 Products
Product Documents for Human DRAK1 Antibody
Product Specific Notices for Human DRAK1 Antibody
For research use only
Loading...
Loading...
Loading...
Loading...