Human DTX1/DTX4 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB7157
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
CyTOF-ready, Immunocytochemistry, Intracellular Staining by Flow Cytometry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 730612
Product Specifications
Immunogen
E. coli-derived recombinant human DTX1/DTX4
Met1-Phe147
Accession # Q86Y01
Met1-Phe147
Accession # Q86Y01
Specificity
Detects human DTX1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, 100%
cross-reactivity with recombinant human DTX4 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human DTX1/DTX4 Antibody
Detection of Human DTX1/DTX4 by Western Blot.
Western blot shows lysates of K562 human chronic myelogenous leukemia cell line and SW13 human adrenal cortex adenocarcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human DTX1/DTX4 Monoclonal Antibody (Catalog # MAB7157) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for DTX1/DTX4 at approximately 67 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
DTX1/DTX4 in K562 Human Cell Line.
DTX1/DTX4 was detected in immersion fixed K562 human chronic myelogenous leukemia cell line using Mouse Anti-Human DTX1/DTX4 Monoclonal Antibody (Catalog # MAB7157) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Detection of DTX1/DTX4 in K562 Human Cell Line by Flow Cytometry.
K562 human chronic myelogenous leukemia cell line was stained with Mouse Anti-Human DTX1/DTX4 Monoclonal Antibody (Catalog # MAB7157, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.Applications for Human DTX1/DTX4 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed K562 human chronic myelogenous leukemia cell line
Sample: Immersion fixed K562 human chronic myelogenous leukemia cell line
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
Sample:
Sample:
| K562 human chronic myelogenous leukemia cell line fixed with paraformaldehyde and permeabilized with saponin. |
Western Blot
2 µg/mL
Sample: K562 human chronic myelogenous leukemia cell line and SW13 human adrenal cortex adenocarcinoma cell line
Sample: K562 human chronic myelogenous leukemia cell line and SW13 human adrenal cortex adenocarcinoma cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: DTX1/DTX4
Additional DTX1/DTX4 Products
Product Documents for Human DTX1/DTX4 Antibody
Product Specific Notices for Human DTX1/DTX4 Antibody
For research use only
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