Human E-Selectin/P-Selectin (CD62E/P) APC-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB6169A

R&D Systems, part of Bio-Techne

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry

Cited:

Bioassay

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # BBIG-E6 (13D5)

View all E-Selectin (CD62E)/P-Selectin (CD62P) Antibodies »

Product Specifications

Immunogen

Activated HUVEC human umbilical vein endothelial cells

Specificity

Detects human E‑Selectin/P‑Selectin (CD62E/P). Binds to COS cells transfected with human E-Selectin or human P-Selectin. It does not bind to CHO cells transfected with human ICAM-1, L-Selectin, PECAM-1 or VCAM-1.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human E-Selectin/P-Selectin (CD62E/P) APC-conjugated Antibody

Detection of E-Selectin/P-Selectin (CD62E/P) antibody in HUVEC Human Cells antibody by Flow Cytometry.

Detection of E‑Selectin/P‑Selectin (CD62E/P) in HUVEC Human Cells by Flow Cytometry.

HUVEC human umbilical vein endothelial cells treated with Recombinant Human TNF-a (Catalog # 210-TA) were stained with Mouse Anti-Human E-Selectin/P-Selectin (CD62E/P) APC-conjugated Monoclonal Antibody (Catalog # FAB6169A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human E-Selectin/P-Selectin (CD62E/P) APC-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: HUVEC human umbilical vein endothelial cells treated with Recombinant Human TNF‑ alpha (Catalog # 210-TA)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: E-Selectin (CD62E)/P-Selectin (CD62P)

E-Selectin (CD62E) and P-Selectin (CD62P) are related (44% amino acid sequence identity) adhesion molecules containing multiple short complement-like repeats and an N-terminal C-type lectin domain in their extracellular regions. E-Selectin is expressed on activated vascular endothelial cells and P-Selectin occurs on activated platelets and endothelial cells.

Alternate Names

Eselectin, PSelectin

Additional E-Selectin (CD62E)/P-Selectin (CD62P) Products

Product Documents for Human E-Selectin/P-Selectin (CD62E/P) APC-conjugated Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human E-Selectin/P-Selectin (CD62E/P) APC-conjugated Antibody

For research use only

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