Human E2F-1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4825
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Mouse
Applications
Validated:
Immunohistochemistry, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human E2F-1
Val264-Phe437
Accession # Q01094
Val264-Phe437
Accession # Q01094
Specificity
Detects human E2F-1 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human E2F-2, -3, -4, -5, -6, -7, and -8 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human E2F-1 Antibody
E2F-1 in Human Placenta.
E2F-1 was detected in immersion fixed paraffin-embedded sections of human placenta using 5 µg/mL Sheep Anti-Human E2F-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4825) overnight at 4 °C. Tissue was stained with the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Human E2F-1 by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and human skin tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human E2F-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4825) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for E2F-1 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.Applications for Human E2F-1 Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human placenta
Sample: Immersion fixed paraffin-embedded sections of human placenta
Western Blot
1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line and human skin tissue
Sample: HeLa human cervical epithelial carcinoma cell line and human skin tissue
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: E2F-1
E2F-1 (adenovirus E2 gene promoter region binding factor 1; also retinoblastoma [RB]-binding protein 3/RBP3) is a 57 - 60 kDa nuclear member of the E2F/DP family of transcription factors. It has a dual function, mediating both cell proliferation and apoptosis in a context-dependent manner. Human E2F-1 is 437 amino acids (aa) in length. It contains a cyclin A: CDK2 binding site (aa 67 - 108), a DNA-binding region (aa 110 - 194), and an RB protein binding motif (aa 409 - 426) that, when engaged, inactivates E2F-1. There is one potential in-frame 23 aa substitution for aa 89 - 111. Over aa 264 - 437, human E2F-1 shows 89% and 80% aa identity to canine and mouse E2F-1, respectively.
Long Name
E2F Transcription Factor 1
Alternate Names
E2F1, PBR3, RBAP-1, RBBP-3, RBP3
Gene Symbol
E2F1
UniProt
Additional E2F-1 Products
Product Documents for Human E2F-1 Antibody
Product Specific Notices for Human E2F-1 Antibody
For research use only
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