Human E2F-2 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5119
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human E2F-2
Glu308-Asn437
Accession # Q14209
Glu308-Asn437
Accession # Q14209
Specificity
Detects human E2F-2 in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human E2F-2 Antibody
Detection of Human E2F-2 by Western Blot.
Western blot shows lysates of HEK293 human embryonic kidney cell line. Gels were loaded with 30 µg of whole cell lysate (WCL), 20 µg of cytoplasmic (Cyto), and 10 µg of nuclear extracts (Nuc). PVDF membrane was probed with 1 µg/mL Goat Anti-Human E2F-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5119) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band for E2F-2 was detected at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human E2F-2 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: HEK293 human embryonic kidney cell line
Sample: HEK293 human embryonic kidney cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: E2F-2
E2F-2 (viral E2-associated factor 2) is a 50‑55 kDa member of the E2F/DP family of transcription factors. It is principally expressed by placenta, and forms a DNA‑activating E2F heterodimeric complex with DP-1 or -2. This complex, when active, promotes cell cycle progression. In quiescent cells, association with the retinoblastoma-tumor suppressor gene product termed pRB suppresses its activity. Human E2F‑2 is 437 amino acids (aa) in length and contains a CDK2 binding region (aa 65‑105), a DNA‑binding domain (aa 107‑196), a dimerization segment (aa 197‑289), a transactivation region (aa 359‑437), and a pRB binding domain (aa 410‑427). There are two potential alternate start sites at Met197 and Met342, and one splice variant that shows a two aa substitution for aa 349‑437. Over aa 308‑437, human E2F-2 is 72% aa identical to mouse E2F-2.
Long Name
E2F Transcription Factor 2
Alternate Names
E2F2
Gene Symbol
E2F2
UniProt
Additional E2F-2 Products
Product Documents for Human E2F-2 Antibody
Product Specific Notices for Human E2F-2 Antibody
For research use only
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