Human E2F-4 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5139
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human E2F-4
Lys178-Asp306
Accession # Q16254
Lys178-Asp306
Accession # Q16254
Specificity
Detects endogenous human E2F-4 in Western bots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human E2F-4 Antibody
Detection of Human E2F-4 by Western Blot.
Western blot shows lysates of HEK293 human embryonic kidney cell line, HeLa human cervical epithelial carcinoma cell line, and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 1 µg/mL of Human E2F-4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5139) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for E2F-4 at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human E2F-4 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: HEK293 human embryonic kidney cell line, HeLa human cervical epithelial carcinoma cell line, and K562 human chronic myelogenous leukemia cell line
Sample: HEK293 human embryonic kidney cell line, HeLa human cervical epithelial carcinoma cell line, and K562 human chronic myelogenous leukemia cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: E2F-4
E2F-4 (viral E2-associated factor 4) is a 50 kDa member of the E2F/DP family of transcription factors. It is ubiquitously and constitutively expressed, and forms a DNA-binding E2F heterodimeric complex with DP-1. This complex, when associated with p130/RBL2 and a series of LIN-numbered proteins, represses cell cycle‑dependent genes during G0 and G1. Human E2F-4 is 413 amino acids (aa) in length and contains a poly-Pro region (aa 9‑12), a DNA-binding domain (aa 16‑85), a dimerization segment (aa 86‑181), poly-Ser region (aa 307‑327), and an RBL1/RBL2 binding domain (aa 390‑407). There is one potential alternate start site at Met261. Over aa 178‑306, human E2F-4 shares 80% and 83% aa identity with mouse and canine E2F-4, respectively.
Long Name
E2F Transcription Factor 4
Alternate Names
E2F4
Gene Symbol
E2F4
UniProt
Additional E2F-4 Products
Product Documents for Human E2F-4 Antibody
Product Specific Notices for Human E2F-4 Antibody
For research use only
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