Human E6AP/UBE3A Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB11498
Recombinant Monoclonal Antibody.
Key Product Details
Species Reactivity
Human
Applications
Simple Western, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Mouse IgG1 Clone # 1077332
Product Specifications
Immunogen
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human E6AP/UBE3A
Met1-Leu875
Accession # Q05086
Met1-Leu875
Accession # Q05086
Specificity
Detects human E6AP/UBE3A in Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human E6AP/UBE3A Antibody
Detection of Human E6AP/UBE3A by Western Blot.
Western Blot shows lysates of Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 2 µg/ml of Mouse Anti-Human E6AP/UBE3A Monoclonal Antibody (Catalog # MAB11498) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for E6AP/UBE3A at approximately 98 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
Detection of Human E6AP/UBE3A by Simple WesternTM.
Simple Western shows lysates of K562 human chronic myelogenous leukemia cell line, loaded at 0.2 mg/ml. A specific band was detected for E6AP/UBE3A at approximately 106 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human E6AP/UBE3A Monoclonal Antibody (Catalog # MAB11498). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Human E6AP/UBE3A Antibody
Application
Recommended Usage
Simple Western
20 µg/mL
Sample: K562 human chronix myelogenous leukemia cell line
Sample: K562 human chronix myelogenous leukemia cell line
Western Blot
2 µg/mL
Sample: Jurkat human acute T cell leukemia cell line
Sample: Jurkat human acute T cell leukemia cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from cell culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: E6AP/UBE3A
References
- Dhananjayan, S.C. et. al. (2006) Mol. Endocrinol. 20:2343.
- Louria-Hayon, I. et. al. (2009) Cell Death Differ. 16:1156.
- Martinez-Noel, G. et. al. (2012) Mol. Cell. Biol. 32:3095.
- Gossan, N.C. et. al. (2014) Nucleic Acids Res. 42:5765.
- Sadikovic, B. et. al. (2014) Hum. Mutat. 35:1407.
- Khatri, N. and H-Y Man. (2019) Front. Mol. Neurosci. 12:109.
Long Name
Human Papillomavirus E6-associated Protein
Alternate Names
ANCR, EPVE6AP, HPVE6A, UBE3A
Gene Symbol
UBE3A
UniProt
Additional E6AP/UBE3A Products
Product Documents for Human E6AP/UBE3A Antibody
Product Specific Notices for Human E6AP/UBE3A Antibody
For research use only
Loading...
Loading...
Loading...
Loading...