Human EDA/Ectodysplasin Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB9222
Key Product Details
Species Reactivity
Validated:
Human
Applications
ELISA Capture (Matched Antibody Pair)
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 174806
Product Summary for Human EDA/Ectodysplasin Antibody
Immunogen
Mouse myeloma cell line NS0-derived recombinant human EDA-A2/Ectodysplasin A2
Ala179-Ser389
Accession # NP_001005609
Ala179-Ser389
Accession # NP_001005609
Specificity
Detects human EDA/Ectodysplasin (EDA-A1 and EDA-A2) in ELISAs. In sandwich immunoassays, approximately 50% cross-reactivity with recombinant mouse EDA is observed, and no cross-reactivity with recombinant human (rh) TNF-alpha, rhXEDAR, or rhEDAR is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Applications for Human EDA/Ectodysplasin Antibody
Application
Recommended Usage
Human EDA-A2/Ectodysplasin A2 Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: EDA/Ectodysplasin
The products of the Ectodysplasin A gene (EDA, also called Tabby) are type II transmembrane proteins that belong to the TNF superfamily. The EDA gene encodes two splice variants, designated EDA-A1 and EDA-A2, that differ only by the deletion of two amino acids in the extracellular domain of EDA-A2. Despite this minor difference, the EDA isoforms display strong receptor specificity. EDA-A1 only binds EDAR, a member of the TNF Receptor superfamily (TNFRSF), whereas EDA-A2 binds to XEDAR, an X-linked TNFRSF member with high homology to EDAR. The extracellular domains of human and mouse EDA-A2 share approximately 94% amino acid sequence identity.
Alternate Names
Tabby, XHED, XLHED
Gene Symbol
EDA
UniProt
Additional EDA/Ectodysplasin Products
Product Documents for Human EDA/Ectodysplasin Antibody
Product Specific Notices for Human EDA/Ectodysplasin Antibody
For research use only
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