Western blot shows lysates of HEK293 human embryonic kidney cell line either mock transfected or transfected with human EDNRB/Endothelin R Type B. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human EDNRB/ Endothelin R Type B Monoclonal Antibody (Catalog # MAB4496) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for EDNRB/Endothelin R Type B at approximately 77 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of EDNRB in HEK293 Human Cell Line Transfected with Human EDNRB and eGFP by Flow Cytometry.

HEK293 human embryonic kidney cell line transfected with (A) human EDNRB or (B) irrelevant protein, and eGFP was stained with Mouse Anti-Human EDNRB Monoclonal Antibody (Catalog # MAB4496) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on Mouse IgG2A Isotype Control (Catalog # MAB003). View our protocol for Staining Membrane-associated Proteins.

EDNRB/Endothelin R Type B in Human Placenta.

EDNRB/Endothelin R Type B was detected in immersion fixed paraffin-embedded sections of human placenta using Mouse Anti-Human EDNRB/Endothelin R Type B Monoclonal Antibody (Catalog # MAB4496) at 1.7 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to smooth muscle. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human EDNRB/Endothelin R Type B Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: HEK293 Human Cell Line Transfected with Human EDNRB and eGFP

Immunohistochemistry

1-25 µg/mL
Sample:

Immersion fixed paraffin-embedded sections of human placenta

Western Blot

0.5 µg/mL
Sample: HEK293 human embryonic kidney cell line transfected with human EDNRB/Endothelin R Type B

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EDNRB/Endothelin R Type B

EDNRB (Endothelin Receptor Type B) is a 50-55 kDa member of the beta-family of rhodopsin receptors. It binds endothelin 1, 2 and 3, and is found on endothelial cells where it mediates vasodilation. Mature human EDNRB is a 7-transmembrane glycoprotein that is 416 amino acids (aa) in length. It contains a 75 aa N-terminal extracellular region (aa 27-101), and a 44 aa C-terminal cytoplasmic domain. There are three EDNRB variants that affect aa 27-101. One shows a 90 aa N-terminal extension, a second shows the same 90 aa N-terminal substitution coupled with a deletion of aa 268-398, and a third shows proteolytic cleavage between Arg64‑Ser65. Over aa 27-101, human EDNRB shares 67% and 97% aa identity with mouse and canine EDNRB, respectively.

Long Name

Endothelin Receptor Type B

Alternate Names

EDNRB, ET-B, ETRB

Entrez Gene IDs

1910 (Human); 13618 (Mouse); 50672 (Rat)

Gene Symbol

EDNRB

UniProt

P24530

Additional EDNRB/Endothelin R Type B Products

Product Documents for Human EDNRB/Endothelin R Type B Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human EDNRB/Endothelin R Type B Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human EDNRB/Endothelin R Type B Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB4496

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all EDNRB/Endothelin R Type B Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human EDNRB/Endothelin R Type B Antibody

Detection of Human EDNRB/Endothelin R Type B by Western Blot.