Human EDNRB/Endothelin R Type B Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4496
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
CyTOF-ready, Flow Cytometry
Cited:
Flow Cytometry
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human EDNRB
Glu27-Lys101
Accession # P24530
Glu27-Lys101
Accession # P24530
Specificity
Detects human EDNRB in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human EDNRB/Endothelin R Type B Antibody
Detection of EDNRB/Endothelin R Type B in A172 Human Cell Line by Flow Cytometry.
A172 human glioblastoma cell line was stained with Human EDNRB/Endothelin R Type B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4496, filled histogram) or isotype control antibody (Catalog # 5-001-A, open histogram), followed by NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL010).
Detection of EDNRB in HEK293 Human Cell Line Transfected with Human EDNRB and eGFP by Flow Cytometry.
HEK293 human embryonic kidney cell line transfected with either (A) human EDNRB or (B) irrelevant protein and eGFP was stained with Sheep Anti-Human EDNRB Affinity Purified Polyclonal Antibody (Catalog # AF4496) followed by Allophycocyanin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0127). Quadrant markers were set based on control antibody staining (Catalog # 5-001-A). View our protocol for Staining Membrane-associated Proteins.Applications for Human EDNRB/Endothelin R Type B Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
0.25 µg/106 cells
Sample: A172 human glioblastoma cell line and HEK293 Human Cell Line Transfected with Human EDNRB and eGFP
Sample: A172 human glioblastoma cell line and HEK293 Human Cell Line Transfected with Human EDNRB and eGFP
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: EDNRB/Endothelin R Type B
EDNRB (Endothelin B Receptor) is a member of the beta-family of rhodopsin receptors. It binds endothelin 1, 2, and 3 and is found on endothelial cells where it mediates vasodilation. Mature human EDNRB is a 7-transmembrane glycoprotein that is 416 amino acids (aa) in length. It contains a 75 aa N-terminal extracellular region (aa 27-101), and a 44 aa C-terminal cytoplasmic domain. There are three EDNRB variants that affect aa 27-101. One shows a 90 aa N-terminal extension, a second shows the same 90 aa N-terminal substitution coupled with a deletion of aa 268-398, and a third shows proteolytic cleavage between Arg64-Ser65. Over aa 27-101, human EDNRB shares 67% and 97% aa sequence identity with mouse and canine EDNRB, respectively.
Long Name
Endothelin Receptor Type B
Alternate Names
EDNRB, ET-B, ETRB
Gene Symbol
EDNRB
UniProt
Additional EDNRB/Endothelin R Type B Products
Product Documents for Human EDNRB/Endothelin R Type B Antibody
Product Specific Notices for Human EDNRB/Endothelin R Type B Antibody
For research use only
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