Sheep IgG (H+L) APC-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # F0127
Conjugate
Catalog #
Key Product Details
Species Reactivity
Sheep
Applications
Flow Cytometry
Label
Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)
Antibody Source
Polyclonal Donkey IgG
Product Specifications
Immunogen
Sheep IgG
Specificity
Sheep IgG specific IgG was purified by sheep IgG (H+L) affinity chromatography and absorbed against human IgG, and human, mouse, bovine, and rabbit serum to eliminate cross-reactivity.
Clonality
Polyclonal
Host
Donkey
Isotype
IgG
Scientific Data Images for Sheep IgG (H+L) APC-conjugated Antibody
Detection of Sheep IgG Primary Antibody by Flow Cytometry
Human peripheral blood granulocytes were stained with Sheep Anti-Human Fce RIa Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6678, filled histogram) or isotype control antibody (Catalog # 5-001-A), open histogram), followed by Allophycocyanin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0127) at 10 µL/106cells.Applications for Sheep IgG (H+L) APC-conjugated Antibody
Application
Recommended Usage
Flow Cytometry
Designed for use as a secondary developing reagent in immunofluorescent assays, such as flow cytometry, where the primary antibody does not have a fluorescent reporter, is of sheep origin, and is of IgG class. The recommended concentration is 10 μL/106 cells.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IgG
R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.
Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.
Long Name
Immunoglobulin G
Alternate Names
Immunoglobulin G, ImmunoglobulinG
Additional IgG Products
Product Documents for Sheep IgG (H+L) APC-conjugated Antibody
Product Specific Notices for Sheep IgG (H+L) APC-conjugated Antibody
For research use only
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