Human EGF Antibody
R&D Systems, part of Bio-Techne | Catalog # AF236
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry, Neutralization, Western Blot
Cited:
ELISA Development, Immunocytochemistry, Immunohistochemistry, Neutralization, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human EGF
Specificity
Detects human EGF in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant rat EGF and 5% cross-reactivity with recombinant mouse EGF is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Human EGF Antibody
Detection of Recombinant Human, Mouse, and Rat EGF by Western Blot.
Western blot shows 100 ng of Recombinant Human EGF (Catalog # 236-EG), Recombinant Mouse EGF (Catalog # 2028-EG) and Recombinant Rat EGF (Catalog # 3214-EG). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human EGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF236) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for EGF at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
EGF in Human Placenta.
EGF was detected in immersion fixed paraffin-embedded sections of human placenta using Goat Anti-Human EGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF236) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to syncytiotrophoblasts. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Cell Proliferation Induced by EGF and Neutralization by Human EGF Antibody.
Recombinant Human EGF (Catalog # 236-EG) stimulates proliferation in the Balb/3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human EGF (2 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human EGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF236). The ND50 is typically 0.040 - 0.480 µg/mL.Applications for Human EGF Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human prostate cancer and human placenta
Sample: Immersion fixed paraffin-embedded sections of human prostate cancer and human placenta
Western Blot
0.1 µg/mL
Sample: Recombinant Human EGF (Catalog # 236-EG)
Sample: Recombinant Human EGF (Catalog # 236-EG)
Neutralization
Measured by its ability to neutralize EGF-induced proliferation in the Balb/3T3 mouse embryonic fibroblast cell line. The Neutralization Dose (ND50) is typically 0.040 - 0.480 µg/mL in the presence of 2 ng/mL Recombinant Human EGF.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: EGF
Long Name
Epidermal Growth Factor
Alternate Names
HOMG4, URG, Urogastrone
Gene Symbol
EGF
Additional EGF Products
Product Documents for Human EGF Antibody
Product Specific Notices for Human EGF Antibody
For research use only
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