Human EGFR Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB1095
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Mouse, Complex Species Category
Applications
Validated:
Western Blot
Cited:
Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Neutralization, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 102618
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human EGFR
Leu25-Ser645
Accession # CAA25240
Leu25-Ser645
Accession # CAA25240
Specificity
Detects human EGFR in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse (rm) EGFR, recombinant human (rh) ErbB2, rmErbB2, rhErbB3, or rhErbB4 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Applications for Human EGFR Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Recombinant Human EGFR (Catalog # 1095-ER) under non-reducing conditions only
Sample: Recombinant Human EGFR (Catalog # 1095-ER) under non-reducing conditions only
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 5 using MAB1095 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: EGFR
Long Name
Epidermal Growth Factor Receptor
Alternate Names
EGF R, ErbB, ErbB1, HER-1
Gene Symbol
EGFR
UniProt
Additional EGFR Products
Product Documents for Human EGFR Antibody
Product Specific Notices for Human EGFR Antibody
For research use only
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