Human EGFR (Research Grade Cetuximab Biosimilar) Biotinylated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB9577B
Recombinant Monoclonal Antibody For Research Use Only
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Flow Cytometry, Immunocytochemistry
Cited:
Flow Cytomery, Flow Cytometry, Functional Assay
Label
Biotin
Antibody Source
Recombinant Monoclonal Human IgG1 Clone # Hu1
Product Specifications
Immunogen
Human EGF R/ErbB1
Specificity
Detects human EGFR based on Cetuximab therapeutic antibody. This non-therapeutic antibody uses the same variable region sequence as the therapeutic antibody Cetuximab. This product is for research use only.
Clonality
Monoclonal
Host
Human
Isotype
IgG1
Scientific Data Images
Detection of EGF R/ErbB1 in A431 human epithelial carcinoma cell line by Flow Cytometry.
A431 human epithelial carcinoma cell line was stained with Human Anti-Human EGF R/ErbB1 (Research Grade Cetuximab Biosimilar) Biotinylated Monoclonal Antibody (Catalog # FAB9577B, filled histogram) or irrelevant biotinylated antibody (open histogram) followed by APC-conjugated Streptavidin (Catalog # F0050). View our protocol for Staining Membrane-associated Proteins.
EGFR in A431 Human Cell Line.
EGFR was detected in unfixed live A431 human epithelial carcinoma cell line using Human Anti-Human EGFR (Research Grade Cetuximab Biosimilar) Biotinylated Monoclonal Antibody (Catalog # FAB9577B) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications
Application
Recommended Usage
Flow Cytometry
10 µL/106 cells
Sample: A431 human epithelial carcinoma cell line
Sample: A431 human epithelial carcinoma cell line
Immunocytochemistry
8-25 µg/mL
Reviewed Applications
Read 1 review rated 4 using FAB9577B in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from cell culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: EGFR
Long Name
Epidermal Growth Factor Receptor
Alternate Names
EGF R, ErbB, ErbB1, HER-1
Gene Symbol
EGFR
Additional EGFR Products
Product Specific Notices
For research use only
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