Human EGR1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF2818
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Avian - Chicken
Applications
Validated:
Immunocytochemistry, Western Blot
Cited:
Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human EGR1
Gln282-Ser433
Accession # P18146
Gln282-Ser433
Accession # P18146
Specificity
Detects human EGR1 in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human EGR1 Antibody
Detection of EGR1 in SK-BR-3 Human Cell Line.
EGR1 was detected in immersion fixed SK-BR-3 human breast cancer cell line using Goat Anti-Human EGR1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2818) at 5 µg/ml for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to the nucleus and the cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human EGR1 Antibody
Application
Recommended Usage
Immunocytochemistry
1.7-15 µg/mL
Sample: Immersion fixed SK-BR-3 human breast cancer cell line
Sample: Immersion fixed SK-BR-3 human breast cancer cell line
Western Blot
0.1 µg/mL
Sample: Recombinant Human EGR1
Sample: Recombinant Human EGR1
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: EGR1
Long Name
Early Growth Response 1
Alternate Names
KROX24, NGFIA, ZNF225
Entrez Gene IDs
1958 (Human)
Gene Symbol
EGR1
UniProt
Additional EGR1 Products
Product Documents for Human EGR1 Antibody
Product Specific Notices for Human EGR1 Antibody
For research use only
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