Human eIF2 alpha Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB3997
Key Product Details
Species Reactivity
Validated:
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2B Clone # 430516
Product Summary for Human eIF2 alpha Antibody
Immunogen
E. coli-derived recombinant human eIF2 alpha
Met1-Asp315
Accession # Q53XC0
Met1-Asp315
Accession # Q53XC0
Specificity
Detects human eIF2 alpha in Western blots.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2B
Scientific Data Images for Human eIF2 alpha Antibody
Detection of Human eIF2 alpha by Western Blot.
Western blot shows lysates of Jurkat human acute T cell leukemia cell line, K562 human chronic myelogenous leukemia cell line, and MCF-7 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human eIF2a Monoclonal Antibody (Catalog # MAB3997) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for eIF2a at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human eIF2 alpha Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Jurkat human acute T cell leukemia cell line, K562 human chronic myelogenous leukemia cell line, and MCF-7 human breast cancer cell line
Sample: Jurkat human acute T cell leukemia cell line, K562 human chronic myelogenous leukemia cell line, and MCF-7 human breast cancer cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: eIF2 alpha
Long Name
Eukaryotic Translation Initiation Factor 2 Alpha
Alternate Names
EIF2S1
Gene Symbol
EIF2S1
UniProt
Additional eIF2 alpha Products
Product Documents for Human eIF2 alpha Antibody
Product Specific Notices for Human eIF2 alpha Antibody
For research use only
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