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Human EMILIN2 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB10590

R&D Systems, part of Bio-Techne
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MAB10590-100
MAB10590-SP

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 1031401

Product Specifications

Immunogen

Human EMILIN2 synthetic peptide

Specificity

Detects human EMILIN2 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human EMILIN2 Antibody

EMILIN2 in THP-1 Human Cell Line.

EMILIN2 was detected in immersion fixed THP-1 human acute monocytic leukemia cell line (left panel; positive staining) and Daudi human Burkitt's lymphoma cell line (right panel; negative staining) using Mouse Anti-Human EMILIN2 Monoclonal Antibody (Catalog # MAB10590) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Human EMILIN2 Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed THP‑1 human acute monocytic leukemia cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EMILIN2

EMILIN (Elastin Microfibril Interface Located Protein) is an elastic fiber-associated glycoprotein.  EMILIN2 is a protein that may be responsible for anchoring smooth muscles to elastin fibers.  It also may be involved in the process that regulates vessel assembly. MRNA expression of EMILIN2 is more restricted compared to EMILIN1 with highest levels being present in fetal heart and adult lung.  EMILIN2 is secreted extracellularly by in vitro-grown cells.

Long Name

Elastin Microfibril Interfacer 2

Alternate Names

EMIL2, FLJ33200, FOAP-10

Entrez Gene IDs

84034 (Human)

Gene Symbol

EMILIN2

Additional EMILIN2 Products

Product Documents for Human EMILIN2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human EMILIN2 Antibody

For research use only

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