Human EMP3 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB4505
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
CyTOF-ready, Intracellular Staining by Flow Cytometry
Cited:
Flow Cytometry, Neutralization
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 439949
Product Specifications
Immunogen
NS0 mouse myeloma cell line transfected with human EMP3
Met1-Glu163
Accession # P54852
Met1-Glu163
Accession # P54852
Specificity
Detects human EMP. Stains human EMP3 cell transfectants but not irrelevant transfectants.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human EMP3 Antibody
Detection of EMP3 in Human PBMCs by Flow Cytometry.
Human peripheral blood mononuclear cells were stained with Mouse Anti-Human EMP3 Monoclonal Antibody (Catalog # MAB4505, filled histogram) or isotype control antibody (MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
Detection of EMP3 in PBMC monocytes by Flow Cytometry.
PBMC monocytes were stained with Mouse Anti-Human EMP3 Monoclonal Antibody (Catalog # MAB4505, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed and permeabilized with Flow Cytometry Fixation Buffer (Catalog # FC004). View our protocol for Staining Intracellular Molecules.Applications for Human EMP3 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
Sample: Human peripheral blood mononuclear cells fixed with paraformaldehyde and permeabilized with saponin and PBMC monocytes fixed and permeabilized with Flow Cytometry Fixation Buffer (Catalog # FC004).
Sample: Human peripheral blood mononuclear cells fixed with paraformaldehyde and permeabilized with saponin and PBMC monocytes fixed and permeabilized with Flow Cytometry Fixation Buffer (Catalog # FC004).
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: EMP3
Epithelial membrane protein 3 (EMP3) is a multipass transmembrane protein in the peripheral myelin protein 22 family. It is expressed as a 20‑25 kDa molecule depending on the degree of glycosylation. EMP3 interacts with the P2X7 purinergic receptor on monocytes. Its dysregulation is associated with glioma and neuroblastoma. Human EMP3 shares 93% aa sequence identity with mouse and rat EMP3.
Long Name
Epithelial Membrane Protein 3
Alternate Names
HNMP-1, YMP
Gene Symbol
EMP3
UniProt
Additional EMP3 Products
Product Documents for Human EMP3 Antibody
Product Specific Notices for Human EMP3 Antibody
For research use only
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