HEK293 human embryonic kidney cell line transfected with either (A) human EMR3 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human EMR3 APC-conjugated Monoclonal Antibody (Catalog # FAB8496A). Quadrant markers were set based on control antibody staining (Catalog # IC0041A). View our protocol for Staining Membrane-associated Proteins.

Detection of EMR3 in Human Peripheral Blood Monocytes by Flow Cytometry.

Human peripheral blood monocytes were stained with Mouse Anti-Human CD14 PE-conjugated Monoclonal Antibody (Catalog # FAB3832P) and either (A) Mouse Anti-Human EMR3 APC-conjugated Monoclonal Antibody (Catalog # FAB8496A) or (B) Mouse IgG_2BAllophycocyanin Isotype Control (Catalog # IC0041A). View our protocol for Staining Membrane-associated Proteins.

Applications for Human EMR3 APC-conjugated Antibody

Application

Recommended Usage

Flow Cytometry

10 µL/10⁶ cells
Sample: Human peripheral blood monocytes and HEK293 human embryonic kidney cell line transfected with human EMR3 and eGFP

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: EMR3

EMR3 is a member of the class B seven-span transmembrane (TM7) receptor family. It is expressed predominantly by cells of the immune system, with highest levels in neutrophils, monocytes and macrophages. It may play a role in myeloid-myeloid interactions during immune and inflammatory responses. EMR3 upregulation in glioblastoma multiforme (GBM) is associated with poor survival and may be a potential therapeutic target.

Long Name

EGF-like Module Containing, Mucin-like, Hormone Receptor-like 3

Alternate Names

egf-like module containing, mucin-like, hormone receptor-like 3, EGF-like module receptor 3, EGF-like module-containing mucin-like hormone receptor-like 3, egf-like module-containing mucin-like receptor 3

Entrez Gene IDs

84658 (Human)

Gene Symbol

ADGRE3

UniProt

NP_115960

Additional EMR3 Products

Product Documents for Human EMR3 APC-conjugated Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human EMR3 APC-conjugated Antibody

For research use only

Citations
Reviews

Spectra Viewer
Flow Cytometry

Human EMR3 APC-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB8496A

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all EMR3 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human EMR3 APC-conjugated Antibody

Detection of EMR3 in HEK293 Human Cell Line Transfected with Human EMR3 and eGFP by Flow Cytometry.