Human Enteropeptidase/Enterokinase Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB1585
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Mouse
Applications
Validated:
Immunoprecipitation, Western Blot
Cited:
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 190034
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Enteropeptidase/Enterokinase
Leu41-His1019
Accession # NP_002763
Leu41-His1019
Accession # NP_002763
Specificity
Detects human Enteropeptidase/Enterokinase in direct ELISAs and Western blots. It recognizes all EK forms containing the heavy chain including the single chain precursor and the mature heterodimer consisting of a heavy and light chain. In Western blots, less than 5% cross‑reactivity with recombinant human (rh) Factor X and rhThrombin is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Applications for Human Enteropeptidase/Enterokinase Antibody
Application
Recommended Usage
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Enteropeptidase/Enterokinase (Catalog # 1585-SE), see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Human Enteropeptidase/Enterokinase (Catalog # 1585-SE), see our available Western blot detection antibodies
Western Blot
1 µg/mL
Sample: Recombinant Human Enteropeptidase/Enterokinase (Catalog # 1585-SE) under non-reducing conditions only
Sample: Recombinant Human Enteropeptidase/Enterokinase (Catalog # 1585-SE) under non-reducing conditions only
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Enteropeptidase/Enterokinase
Enterokinase (EK), also called enteropeptidase and TMPRSS15 (gene name), initiates activation of pancreatic proteases by converting trypsinogen to trypsin, which in turn activates chymotrypsin, carboxypeptidases and elastases. Located in intestinal brush border, it is a disulfide bond-linked dimer of the heavy and light chains, which are derived from the same single-chain precursor. The heavy chain consists of a short cytoplamic tail, a transmembrane, a SEA, a SRCR, a MAM, two CUB and two LDL-receptor class A domains. The light chain contains the catalytic domain of trypsin-like serine proteases.
Alternate Names
Enterokinase, ENTK, PRSS7, TMPRSS15
Entrez Gene IDs
5651 (Human)
Gene Symbol
TMPRSS15
UniProt
Additional Enteropeptidase/Enterokinase Products
Product Documents for Human Enteropeptidase/Enterokinase Antibody
Product Specific Notices for Human Enteropeptidase/Enterokinase Antibody
For research use only
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