Human Epiregulin Alexa Fluor® 350-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # IC14251U
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Intracellular Staining by Flow Cytometry
Label
Alexa Fluor 350 (Excitation = 346 nm, Emission = 442 nm)
Antibody Source
Monoclonal Mouse IgG2B Clone # 183629
Product Specifications
Immunogen
E. coli-derived recombinant human Epiregulin
Val63-Leu108
Accession # O14944
Val63-Leu108
Accession # O14944
Specificity
Detects human Epiregulin in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) Betacellulin, rhEGF, recombinant mouse Epiregulin, or rhHB-EGF is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Applications for Human Epiregulin Alexa Fluor® 350-conjugated Antibody
Application
Recommended Usage
Intracellular Staining by Flow Cytometry
0.25-1 µg/106 cells
Sample: A549 human lung carcinoma cell line fixed with paraformaldehyde and permeabilized with saponin
Sample: A549 human lung carcinoma cell line fixed with paraformaldehyde and permeabilized with saponin
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Epiregulin
Epiregulin is a member of the EGF family of growth factors which includes, among others, epidermal growth factor (EGF), transforming growth factor (TGF)-alpha, amphiregulin (ARG), HB (heparin-binding)-EGF, betacellulin, and the various heregulins. All EGF family members are synthesized as transmembrane precursors and are converted to soluble forms by proteolytic cleavage. Epiregulin was originally purified from the mouse fibroblast-derived tumor cell line NIH3T3/T7 (1). The human epiregulin cDNA encodes a 169 amino acid (aa) residues transmembrane precursor with a 29 aa signal peptide, a 21 aa transmembrane domain and a 21 aa cytoplasmic domain. The putative soluble mature Epiregulin comprising the EGF-like domain (aa residues 64‑104) is formed by proteolytic removal of the propeptide regions (2). There is 85% aa sequence homology between human and mouse epiregulins. Epiregulin is expressed primarily in the placenta and macrophages (3). High level expression has also been detected in various carcinomas. Epiregulin specifically binds EGFR (ErbB1) and ErbB4 but not ErbB2 and ErbB3. It activates the homodimers of both ErbB1 and ErbB4. In addition, epiregulin can also activate all possible heteromeric combinations of the four ErbB family members (4). Epiregulin stimulates the proliferation of fibroblasts, smooth muscle cells and hepatocytes. It has been shown to be an autocrine growth factor for epidermal keratinocytes as well as mesangial cells (5, 6). Epiregulin has also been shown to inhibit growth of several epithelial tumor cells. In addition, Epiregulin has been implicated in the implantation process during pregnancy (7).
References
- Toyoda, H. et al. (1995) J. Biol. Chem. 270:7495.
- Toyoda, H. et al. (1997) Biochem. J. 326:69.
- Komurasaki, T. et al. (1997) Oncogene 15:2841.
- Shelly, M. et al. (1998) J. Biol. Chem. 273:10496.
- Shirakata, Y. et al. (2000) J. Biol. Chem. 275:5748.
- Mishre, R. et al. (2002) Am. J. Physiol. Renal. Physiol. 283:F1151.
- Das, S.K. et al. (1997) Dev. Biol. 190:178.
Alternate Names
EREG
Gene Symbol
EREG
UniProt
Additional Epiregulin Products
Product Documents for Human Epiregulin Alexa Fluor® 350-conjugated Antibody
Product Specific Notices for Human Epiregulin Alexa Fluor® 350-conjugated Antibody
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
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