Human ER alpha/NR3A1 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB57153

Recombinant Monoclonal Antibody.
R&D Systems, part of Bio-Techne
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MAB57153-100
MAB57153-SP
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry, Immunohistochemistry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2424B

View all ER alpha/NR3A1 Antibodies »

Product Summary for Human ER alpha/NR3A1 Antibody

Immunogen

E. coli-derived recombinant human ER alpha/NR3A1
Met1-Gln116
Accession # P03372

Specificity

Detects human ER alpha/NR3A1 in direct ELISAs.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human ER alpha/NR3A1 Antibody

ERa/NR3A1 antibody in T47D and WM-115 Human Cell Lines by Immunocytochemistry (ICC).

ER alpha/NR3A1 in T47D and WM-115 Human Cell Lines.

ERa/NR3A1 was detected in immersion fixed T47D human breast cancer cell line (positive stain) and WM-115 human malignant melanoma cell line (negative stain) using Rabbit Anti-Human ERa/NR3A1 Monoclonal Antibody (Catalog # MAB57153) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cell nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
ERa/NR3A1 antibody in Human Breast Cancer Tissue by Immunohistochemistry (IHC-P).

ER alpha/NR3A1 in Human Breast Cancer Tissue.

ERa/NR3A1 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Rabbit Anti-Human ERa/NR3A1 Monoclonal Antibody (Catalog # MAB57153) at 10 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell nuclei. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human ER alpha/NR3A1 Antibody

Application
Recommended Usage

Immunocytochemistry

3-25 µg/mL
Sample: Immersion fixed T47D human breast cancer cell line and WM-115 human malignant melanoma cell line

Immunohistochemistry

10-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human breast cancer tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using MAB57153 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ER alpha/NR3A1

ER alpha (Estrogen receptor alpha; also Estradiol receptor and NR3A1) is a 65-70 kDa member of the NR3 subfamily, nuclear hormone receptor family of proteins. It is widely expressed, and serves as a strong activator of estrogen-responsive genes. ER alpha is normally quiescent and bound to heat-shock proteins and immunophilins. Following beta-estradiol binding, it becomes activated, either homodimerizes or heterodimerizes with ER beta, and binds to DNA with multiple coactivators. Human ER alpha is 595 amino acids (aa) in length.  It contains a DNA binding region (aa 185-250), three NLSs (aa 256-260; 266-271; 299-303), a steroid-binding site (aa 351-543), a dimerization motif (aa 497-518), and an O-GlcNAc attachment around Thr575. Major phosphorylation sites exist at Tyr537, Ser167 and Ser118. Multiple splice forms exist. There is an 80 kDa isoform that shows a substitution (duplication) of aa 412-517 for Asp411, a second isoform with a deletion of aa 255-366, a third isoform with a deletion of aa 152-412, and a fourth isoform that shows a Thr substitution for aa 152-595. Human ER alpha is only 46% aa identical to human ER beta. Over aa 1-116, human ER alpha shares 85% aa identity with mouse ER alpha.

Long Name

Estrogen Receptor alpha

Alternate Names

ESR1, NR3A1

Entrez Gene IDs

2099 (Human)

Gene Symbol

ESR1

UniProt

P03372

Additional ER alpha/NR3A1 Products

Product Documents for Human ER alpha/NR3A1 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Human ER alpha/NR3A1 Antibody

For research use only

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