Human ER alpha/NR3A1 Antibody

R&D Systems, part of Bio-Techne | Catalog # PP-H4624-00

R&D Systems, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Direct ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # H4624

View all ER alpha/NR3A1 Antibodies »

Product Specifications

Immunogen

Recombinant human ER alpha/NR3A1
aa 2-180

Specificity

This antibody specifically recognizes human ER alpha and cross-reacts with mouse and rat ER alpha. This antibody does not recognize human ER beta. Not yet tested in other species.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Applications for Human ER alpha/NR3A1 Antibody

Application
Recommended Usage

Direct ELISA

This antibody can be used at 0.3 μg/mL with the appropriate secondary reagents to detect human ER alpha.

Immunohistochemistry

This antibody can be used at 10 - 20 μg/mL with the appropriate secondary reagents to detect human ER alpha.

Immunoprecipitation

Optimal dilutions should be determined by each laboratory.

Western Blot

This antibody can be used at 1 μg/mL with the appropriate secondary reagents to detect human ER alpha.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

A liquid formulation in physiologic saline with 0.1% NaN3.

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

This antibody is stable for greater than six months when held at -20 °C in a manual defrost freezer or at -70 °C. Upon thawing, the antibody can be stored at 2-8 °C for at least 1 month without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Background: ER alpha/NR3A1

Estrogen Receptor alpha (ER alpha, ER; NR3A1) is a member of the Steroid Receptor family. The natural ligand for ER is the classic estrogenic compound 17 beta-estradiol. ER alpha is expressed in a wide variety of tissues such as bones, uterus, bladder, ovary, prostate, testis, epididymis, kidney, breast, heart, vessel wall, pituitary and hypothalamus. ER has a variety of central physiological roles, including those involved in maintenance of the reproductive, cardiovascular, musculoskeletal and central nervous systems. ER alpha has been shown to form homodimers as well as heterodimers with ER beta. Both ER alpha and ER beta can give rise to numerous isoforms.

Long Name

Estrogen Receptor alpha

Alternate Names

ESR1, NR3A1

Entrez Gene IDs

2099 (Human)

Gene Symbol

ESR1

Additional ER alpha/NR3A1 Products

Product Documents for Human ER alpha/NR3A1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human ER alpha/NR3A1 Antibody

For research use only

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