Human ERK3 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF3196
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human ERK3
Met344-Asn721
Accession # Q16659
Met344-Asn721
Accession # Q16659
Specificity
Detects endogenous human ERK3 in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human ERK3 Antibody
Detection of Human ERK3 by Western Blot.
Western blot shows lysates of MDA-MB-468 human breast cancer cell line, U937 human histiocytic lymphoma cell line, and Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Human ERK3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3196) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for ERK3 at approximately 105 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.Applications for Human ERK3 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: MDA-MB-468 human breast cancer cell line, U937 human histiocytic lymphoma cell line, and Raji human Burkitt's lymphoma cell line
Sample: MDA-MB-468 human breast cancer cell line, U937 human histiocytic lymphoma cell line, and Raji human Burkitt's lymphoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ERK3
Extracellular signal-regulated kinase 3 (ERK3) is a serine/threonine protein kinase also known as mitogen-activated protein kinase 6 (MAPK6) and p97MAPK. Unlike ERK1 and ERK2, ERK3 lacks the phosphoacceptor sequence T-E-Y in its activation loop. This kinase also possesses a unique C-terminal extension that is missing in traditional MAPKs. Highly unstable, ERK3 is rapidly degraded following N-terminal ubiquitination.
Long Name
Extracellular Signal-regulated Kinase 3
Alternate Names
MAPK6, p97MAPK
Gene Symbol
MAPK6
UniProt
Additional ERK3 Products
Product Documents for Human ERK3 Antibody
Product Specific Notices for Human ERK3 Antibody
For research use only
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