Human ERMAP Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4928
Key Product Details
Species Reactivity
Human
Applications
CyTOF-ready, Flow Cytometry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human ERMAP
His30-Ala155
Accession # Q96PL5
His30-Ala155
Accession # Q96PL5
Specificity
Detects human ERMAP in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human ERMAP Antibody
Detection of ERMAP in K562 Human Cell Line by Flow Cytometry.
K562 human chronic myelogenous leukemia cell line was stained with Goat Anti-Human ERMAP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4928, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).Applications for Human ERMAP Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
2.5 µg/106 cells
Sample: K562 human chronic myelogenous leukemia cell line
Sample: K562 human chronic myelogenous leukemia cell line
Western Blot
0.1 µg/mL
Sample: Recombinant Human ERMAP
Sample: Recombinant Human ERMAP
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ERMAP
ERMAP (erythrocyte membrane-associated protein; also Scianna blood group antigen) is a 60‑66 kDa member of the BTN/MOG family, Ig-Superfamily of proteins. It is expressed on erythrocytes and erythrocyte precursors and likely serves as a cell adhesion molecule. Mature human ERMAP is a 446 amino acid (aa) type I transmembrane glycoprotein. It possesses a 126 aa extracellular domain (ECD) (aa 30‑155) that contains one V-type Ig-like domain (aa 30‑140). Single aa changes at Gly35, Glu47, Gly57, Pro60 and Arg81 generate distinct antigens of the Scianna blood group. There is one alternate start site at Met91 and a splice variant that shows an 11 aa substitution for aa 103‑475. Over aa 30‑155, human ERMAP is 56% aa identical to mouse ERMAP. In contrast to human ERMAP ECD, mouse REMAP ECD contains one extra Ig-like domain.
Long Name
Erythroblast Membrane-associated Protein
Alternate Names
RD, SC
Gene Symbol
ERMAP
UniProt
Additional ERMAP Products
Product Documents for Human ERMAP Antibody
Product Specific Notices for Human ERMAP Antibody
For research use only
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