Human Erythropoietin/EPO Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB287
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Rat, Hamster - Cricetulus (Chinese Hamster)
Applications
Validated:
Neutralization
Cited:
ELISA Development (Capture), Immunocytochemistry, Neutralization, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 9C21D11
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human Erythropoietin/EPO
Accession # P01588
Accession # P01588
Specificity
Detects human Erythropoietin/EPO.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Human Erythropoietin/EPO Antibody
Cell Proliferation Induced by Erythropoietin/EPO and Neutralization by Human Erythropoietin/EPO Antibody.
Recombinant Human Erythropoietin/EPO (Tissue Culture Grade) (Catalog # 287-TC) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human Erythropoietin/EPO (Tissue Culture Grade) (0.3 units/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human Erythropoietin/EPO Monoclonal Antibody (Catalog # MAB287). The ND50 is typically 0.5-1.5 µg/mL.Applications for Human Erythropoietin/EPO Antibody
Application
Recommended Usage
Neutralization
Measured by its ability to neutralize Erythropoietin/EPO-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND50) is typically 0.5-1.5 µg/mL in the presence of 0.3 units/mL Recombinant Human Erythropoietin/EPO (Tissue Culture Grade).
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Erythropoietin/EPO
Erythropoietin (EPO) a glycoprotein produced primarily by the kidney, is the principal factor that regulates erythropoiesis by stimulating the proliferation and differentiation of erythroid progenitor cells.
Alternate Names
ECYT5, EPO, MVCD2
Gene Symbol
EPO
UniProt
Additional Erythropoietin/EPO Products
Product Documents for Human Erythropoietin/EPO Antibody
Product Specific Notices for Human Erythropoietin/EPO Antibody
For research use only
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