Human FABP8/M-FABP Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB5866
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 631306
Product Specifications
Immunogen
E. coli-derived recombinant human FABP8/M‑FABP
Ser2-Val132
Accession # P02689
Ser2-Val132
Accession # P02689
Specificity
Detects human FABP8 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human FABP1, 2, 3, 4, 5, 6, 7, or 9 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human FABP8/M-FABP Antibody
Detection of Human FABP8/M‑FABP by Western Blot
Western blot shows lysates of human brain (cerebellum) tissue. PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human FABP8/M-FABP Monoclonal Antibody (Catalog # MAB5866) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for FABP8/M-FABP at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
FABP8/M‑FABP in Human Spinal Cord.
FABP8/M-FABP was detected in immersion fixed paraffin-embedded sections of human spinal cord using using Mouse Anti-Human FABP8/ M-FABP Mono-clonal Antibody (Catalog # MAB5866) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counter-stained with hematoxylin (blue). Specific staining was localized to white matter. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human FABP8/M-FABP Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human spinal cord
Sample: Immersion fixed paraffin-embedded sections of human spinal cord
Western Blot
2 µg/mL
Sample: Human brain (cerebellum) tissue
Sample: Human brain (cerebellum) tissue
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FABP8/M-FABP
FABP8 (fatty acid binding protein-8; also M [myelin]-FABP, P2 and PMP2) is a 15 kDa (predicted) member of the fatty acid binding protein family, calycin superfamily of molecules. It is found in Schwann cells, presumably on the cytoplasmic face of the plasma membrane where it may contribute to fatty acid transport across myelin. Functionally, FABP8 has a high affinity for U-shaped fatty acids such as oleic and palmitic acid. Human FABP8 is 132 amino acids (aa) in length and exhibits two layers of antiparallel beta-strands that envelope a hydrophobic pocket for lipid binding. Arg107 plus Arg127-Ile128-Tyr129 participate in fatty acid binding. Full length human FABP8 shares 87% and 95% aa identity with mouse and rabbit FABP9, respectively.
Long Name
Fatty Acid-Binding Protein 8
Alternate Names
M-FABP, MFABP, MP2, PMP2
Gene Symbol
PMP2
UniProt
Additional FABP8/M-FABP Products
Product Documents for Human FABP8/M-FABP Antibody
Product Specific Notices for Human FABP8/M-FABP Antibody
For research use only
Loading...
Loading...
Loading...
Loading...