Human FABP8/M-FABP Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5866
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human FABP8
Ser2-Val132
Accession # P02689
Ser2-Val132
Accession # P02689
Specificity
Detects human FABP8 in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross-reactivity with recombinant human (rh) FABP2, 3, 4, 5, 6, 7 and 9 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human FABP8/M-FABP Antibody
FABP8 in Human Spinal Cord.
FABP8 was detected in immersion fixed paraffin-embedded sections of human spinal cord using 15 µg/mL Human FABP8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5866) overnight at 4 °C. Tissue was stained with the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
FABP8 in Human Brain.
FABP8 was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Human FABP8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5866) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Human FABP8 by Western Blot.
Western blot shows lysates of human hippocampal and rat spinal cord tissue. PVDF membrane was probed with 1 µg/mL of Human FABP8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5866) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for FABP8 at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 7.Applications for Human FABP8/M-FABP Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human spinal cord and cortex
Sample: Immersion fixed paraffin-embedded sections of human spinal cord and cortex
Western Blot
1 µg/mL
Sample: Human hippocampal and rat spinal cord tissue
Sample: Human hippocampal and rat spinal cord tissue
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FABP8/M-FABP
FABP8 (fatty acid binding protein-8; also M [myelin]-FABP, P2 and PMP2) is a 15 kDa (predicted) member of the fatty acid binding protein family, calycin superfamily of molecules. It is found in Schwann cells, presumably on the cytoplasmic face of the plasma membrane where it may contribute to fatty acid transport across myelin. Functionally, FABP8 has a high affinity for U-shaped fatty acids such as oleic and palmitic acid. Human FABP8 is 132 amino acids (aa) in length and exhibits two layers of anti‑parallel beta-strands that envelope a hydrophobic pocket for lipid binding. Arg107 plus Arg127-Ile128-Tyr129 participate in fatty acid binding. Full length human FABP8 shares 87% and 95% aa identity with mouse and rabbit FABP8, respectively.
Long Name
Fatty Acid-Binding Protein 8
Alternate Names
M-FABP, MFABP, MP2, PMP2
Gene Symbol
PMP2
UniProt
Additional FABP8/M-FABP Products
Product Documents for Human FABP8/M-FABP Antibody
Product Specific Notices for Human FABP8/M-FABP Antibody
For research use only
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