Human FACA/FANCA Antibody
R&D Systems, part of Bio-Techne | Catalog # AF6026
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human FACA/FANCA
Tyr35-Arg142
Accession # O15360
Tyr35-Arg142
Accession # O15360
Specificity
Detects human FACA/FANCA in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse FANCA, recombinant human (rh) FANCD2, rhFANCE, rhFANCF, rhFANCG, and rhFANCI is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human FACA/FANCA Antibody
Detection of Human FACA/FANCA by Western Blot.
Western blot shows lysates of MOLT-4 human acute lymphoblastic leukemia cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human FACA/FANCA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6026) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for FACA/FANCA at approximately 160 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.Applications for Human FACA/FANCA Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: MOLT‑4 human acute lymphoblastic leukemia cell line
Sample: MOLT‑4 human acute lymphoblastic leukemia cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FACA/FANCA
FACA (Fanconi anemia group A) is a 160‑165 kDa protein that belongs to Fanconi anemia protein group I. FACA is widely expressed, activated by phosphorylation, partners with FANCG, and participates in the formation of an eight subunit Fanconi anemia core complex that ubiquitinates FANCD2 and FANCI. Ultimately, this contributes to the reactivation of stalled DNA repair replication forks. Human FACA is 1455 amino acids (aa) in length. It contains one NLS (aa 18‑34) and four phosphorylation sites at Ser849, 850, 858 and 1449. There are four potential splice variants. One shows a premature truncation after Cys297, a second contains a six aa substitution for aa 297‑1455, a third shows a deletion of aa 143‑174 accompanied by truncation after Cys297, and a fourth exhibits an alternative start site at Met528, accompanied by a 35 aa substitution for aa 1390‑1455. Over aa 35‑142, human FACA shares 61% aa identity with mouse FACA.
Long Name
Fanconi Anemia, Complementation Group A
Alternate Names
FAA, FAH, FANCA, FANCH
Gene Symbol
FANCA
UniProt
Additional FACA/FANCA Products
Product Documents for Human FACA/FANCA Antibody
Product Specific Notices for Human FACA/FANCA Antibody
For research use only
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