Human FADD Antibody
R&D Systems, part of Bio-Techne | Catalog # AF2938
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human FADD
Met1-Ser208
Accession # Q13158
Met1-Ser208
Accession # Q13158
Specificity
Detects endogenous human FADD in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human FADD Antibody
Detection of Human FADD by Western Blot.
Western blot shows lysates of A431 human epithelial carcinoma cell line, Ramos and Raji human Burkitt's lymphoma cell lines, and MCF-7 human breast cancer cell line. PVDF membrane was probed with 0.5 µg/mL of Human FADD Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2938) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for FADD at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.Applications for Human FADD Antibody
Application
Recommended Usage
Western Blot
0.5 µg/mL
Sample: A431 human epithelial carcinoma cell line, Ramos and Raji human Burkitt's lymphoma cell lines, and MCF-7 human breast cancer cell line
Sample: A431 human epithelial carcinoma cell line, Ramos and Raji human Burkitt's lymphoma cell lines, and MCF-7 human breast cancer cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FADD
Fas-Associating protein with Death Domain (FADD), also known as MORT1, is a 25 kDa adaptor protein that mediates signaling of death domain containing TNF receptor superfamily members. FADD interacts with the death domain of FAS (CD95) and TNFR-1 and recruits procaspase-8 to the Fas signaling complex, initiating caspase activation and apoptosis.
Long Name
Fas-associated Death Domain Protein
Alternate Names
MORT1
Entrez Gene IDs
8772 (Human)
Gene Symbol
FADD
UniProt
Additional FADD Products
Product Documents for Human FADD Antibody
Product Specific Notices for Human FADD Antibody
For research use only
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