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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Intracellular Staining by Flow Cytometry

Cited:

Flow Cytometry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Monoclonal Mouse IgG2B Clone # 308420

Product Specifications

Immunogen

NS0 mouse myeloma cell line transfected with human FATP1
Met1-Leu646
Accession # Q6PCB7

Specificity

Detects human FATP1 in cell transfectants but not non-transfected parent cells in flow cytometry.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human FATP1 PE-conjugated Antibody

Detection of FATP1 antibody in HT-29 Human Cell Line antibody by Flow Cytometry.

Detection of FATP1 in HT‑29 Human Cell Line by Flow Cytometry.

HT-29 human colon adenocarcinoma cell line was stained with Mouse Anti-Human FATP1 PE-conjugated Monoclonal Antibody (Catalog # IC3304P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). View our protocol for Staining Intracellular Molecules.

Applications for Human FATP1 PE-conjugated Antibody

Application
Recommended Usage

Intracellular Staining by Flow Cytometry

10 µL/106 cells
Sample: HT‑29 human colon adenocarcinoma cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005).  
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: FATP1

FATP1 (Fatty Acid Transport Protein 1), also known as SLC27A1, is a 63-64 kDa protein that belongs to the ATP-dependent AMP-binding enzyme family. A type I transmembrane protein, FATP1 is one of six mammalian FATPs that catalyze an ATP-dependent esterification of long-chain fatty acids. FATP1 is expressed in cells with high rates of fatty acid uptake for storage of energy, principally in brown and white adipocytes, plus skeletal muscle myocytes. FATP1 likely functions as a homodimer, and is found in multiple locations such as cytoplasm, in intracellular membranes, and in the plasma membrane in response to insulin (in adipocytes). Full length FATP1 (amino acids 1-646) shares 89% amino acid sequence identity with mouse FATP1.

Long Name

Fatty Acid Transport Protein 1

Alternate Names

SLC27A1

Entrez Gene IDs

376497 (Human); 26457 (Mouse); 94172 (Rat)

Gene Symbol

SLC27A1

UniProt

Additional FATP1 Products

Product Documents for Human FATP1 PE-conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human FATP1 PE-conjugated Antibody

For research use only

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